If creating v2 sample sheets for devices besides the ones mentioned, validate the final sample sheet has the i5 index in the right orientation as described earlier mentioned.
Refer to Index two (i5) Orientation To learn more on how to enter i5 bases on the sample sheet in forward or reverse enhance orientation.
This means that these program Focus on the assumption that the i5 sequence is usually while in the forward orientation and will quickly build the reverse enhance on the sequences furnished for the right instrument type.
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For Area Run Supervisor (LRM), input the sequences in the reverse enhance when establishing the operate so the program routinely creates the forward orientation as wanted for Evaluation. This is often the other of nearly all of our steering and it is just for the MiniSeq Fast kits.
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For runs uploaded manually (i.e., an Illumina Instrument just isn't being used to make the sample sheet), the index orientation will depend on which instrument that you are applying. Remember to begin to see the Index Orientation Table for info on how you can enter the i5 sequence depending on the instrument and application utilized.
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